Journal: Hormones & Cancer

Article Title: Quantitative Immunohistochemical Analysis and Prognostic Significance of TRPS-1, a New GATA Transcription Factor Family Member, in Breast Cancer

doi: 10.1007/s12672-010-0008-8

Figure Lengend Snippet: TRPS-1 expression in breast cancer and other common female cancers

Article Snippet: The ovarian cancer, endometrial cancer, and colorectal cancer tissue microarrays (TMA) were acquired from Cybrdi, Inc. (Rockville, MD, USA).

Techniques: Expressing

Journal: The Journal of Clinical Investigation

Article Title: APOBEC3G promotes liver metastasis in an orthotopic mouse model of colorectal cancer and predicts human hepatic metastasis

doi: 10.1172/JCI45008

Figure Lengend Snippet: (A) Flow chart of in vivo selection process of colorectal cancer cell metastasis to the liver. The nonmetastatic colorectal cancer cells (SW480) and those with only lymph node metastasis (SW620) were inoculated into the colon of nude mice. Liver tumors were detected by the IVIS Imaging System (Caliper Life Sciences), and tumor cells (L-1 and L-2) were then isolated from the liver lesions and reinoculated to confirm their metastatic phenotype. (B) Representative images of the liver metastases (upper panels) and IVIS luciferase images in mice inoculated with colorectal cancer cells (lower panels). The hepatic metastasis rate of colorectal cancer cells is indicated at the bottom. *P < 0.05; **P < 0.01 compared with SW480 and SW620 cells. (C) Representative immunohistochemistry staining of colorectal primary tumor and hepatic metastases from the orthotopic transplanted nude mice. Scale bars: 50 μm. (D) Gene expression profiles reveal a set of 68 genes that are highly expressed in L-1 and L-2 cells as compared with SW480 and SW620 cells. (E) Immunoblotting analysis of gene expression levels in SW480, SW620, L-1, and L-2 cells.

Article Snippet: Colorectal cancer tissue microarray slides (COC1502 and A203) were purchased from Pantomics and AccuMAX.

Techniques: In Vivo, Selection, Imaging, Isolation, Luciferase, Immunohistochemistry, Staining, Expressing, Western Blot

Journal: The Journal of Clinical Investigation

Article Title: APOBEC3G promotes liver metastasis in an orthotopic mouse model of colorectal cancer and predicts human hepatic metastasis

doi: 10.1172/JCI45008

Figure Lengend Snippet: (A) Images show the invasion activity of the hepatic metastasis L-1 cell line with knocked down group A and group B genes using retroviral shRNAs (si-4A, si-4B, upper panel). Cells migrating to the lower side of the Transwell filter were counted per HPF in 3 fields (lower panel). Each assay was repeated at least twice. Original magnification, ×200. *P < 0.05. (B) Images show the migration activity of the hepatic metastases L-1 cell line with knocked down group A genes using wound healing assay (upper panel). The distance of cell migration was calculated at 3 locations (lower panel). Con, control. (C) Representative images of the IVIS luciferase signal in the mice inoculated with colorectal cancer cells preinfected with 2 groups of retroviral shRNAs. The hepatic metastasis rate is indicated at the bottom. *P < 0.05 compared with L-1–control cells. (D) Invasion activity of the hepatic metastasis L-1 cell line overexpressing the group A genes. (E) Migration activity of the hepatic metastases L-1 cell line overexpressing the group A genes. (F) Representative images of the luciferase signal in mice inoculated with colorectal cancer cells expressing the group A genes. The hepatic metastasis rate is indicated at the bottom. *P < 0.05 compared with SW620-control cells. Bars show the mean value of the representative results from 3 experiments, each conducted in duplicate (± SD).

Article Snippet: Colorectal cancer tissue microarray slides (COC1502 and A203) were purchased from Pantomics and AccuMAX.

Techniques: Activity Assay, Migration, Wound Healing Assay, Luciferase, Expressing

Journal: The Journal of Clinical Investigation

Article Title: APOBEC3G promotes liver metastasis in an orthotopic mouse model of colorectal cancer and predicts human hepatic metastasis

doi: 10.1172/JCI45008

Figure Lengend Snippet: (A) Protein expression levels of 4 genes were detected by immunoblotting in nonmetastatic human colorectal cancer samples and human colorectal cancer samples with hepatic metastases. Met, metastatic. (B) Representative images of protein expression levels of 4 genes in nonmetastatic human colorectal cancer samples and human colorectal cancer with hepatic metastases using immunohistochemistry staining. Scale bar: 50 μm.

Article Snippet: Colorectal cancer tissue microarray slides (COC1502 and A203) were purchased from Pantomics and AccuMAX.

Techniques: Expressing, Western Blot, Immunohistochemistry, Staining

Journal: The Journal of Clinical Investigation

Article Title: APOBEC3G promotes liver metastasis in an orthotopic mouse model of colorectal cancer and predicts human hepatic metastasis

doi: 10.1172/JCI45008

Figure Lengend Snippet: Immunohistochemistry analysis of protein expression in colorectal cancer using bivariate tests

Article Snippet: Colorectal cancer tissue microarray slides (COC1502 and A203) were purchased from Pantomics and AccuMAX.

Techniques: Immunohistochemistry, Expressing

Journal: The Journal of Clinical Investigation

Article Title: APOBEC3G promotes liver metastasis in an orthotopic mouse model of colorectal cancer and predicts human hepatic metastasis

doi: 10.1172/JCI45008

Figure Lengend Snippet: Summary of immunohistochemistry analysis of protein expression in colorectal cancer using multiple logistic regression

Article Snippet: Colorectal cancer tissue microarray slides (COC1502 and A203) were purchased from Pantomics and AccuMAX.

Techniques: Immunohistochemistry, Expressing

Journal: The Journal of Clinical Investigation

Article Title: APOBEC3G promotes liver metastasis in an orthotopic mouse model of colorectal cancer and predicts human hepatic metastasis

doi: 10.1172/JCI45008

Figure Lengend Snippet: Representative staining of APOBEC3G and MMP2 in colorectal cancer samples. Case 1 is a representative specimen with a low level or no expression of APOBEC3G and MMP2; case 2 is a representative specimen with a high level of APOBEC3G and MMP2. Scale bar: 50 μm.

Article Snippet: Colorectal cancer tissue microarray slides (COC1502 and A203) were purchased from Pantomics and AccuMAX.

Techniques: Staining, Expressing

Journal: The Journal of Clinical Investigation

Article Title: APOBEC3G promotes liver metastasis in an orthotopic mouse model of colorectal cancer and predicts human hepatic metastasis

doi: 10.1172/JCI45008

Figure Lengend Snippet: APOBEC3G expression is correlated with MMP2 expression and tumor stage in primary colorectal cancer samples

Article Snippet: Colorectal cancer tissue microarray slides (COC1502 and A203) were purchased from Pantomics and AccuMAX.

Techniques: Expressing

Journal: EBioMedicine

Article Title: Type Iγ phosphatidylinositol phosphate kinase promotes tumor growth by facilitating Warburg effect in colorectal cancer

doi: 10.1016/j.ebiom.2019.05.015

Figure Lengend Snippet: High PIPKIγ expression level predicts a poor clinical outcome in colorectal cancer. (A) Real-time qPCR analysis of the mRNA level of PIPKIγ in colorectal cancer cell lines and the normal colonic epithelial cell NCM460. (B) Cell lysates of indicated cells were collected for immunoblotting analyses using PIPKIγ antibody; β-actin was loaded as a control. (C) IHC analysis was performed in a tissue microarray containing 76 matched tumor and non-tumor colorectal cancer tissues. Representative images of PIPKIγ and its expression intensity in non-tumor and tumor tissues were shown. Scale bar: 100 μm. (D) Kaplan-Meier analyses of overall survival of individuals with colorectal cancer based on PIPKIγ protein expression level. (E) Kaplan-Meier analyses of overall survival in colon adenocarcinoma (COAD) and rectal adenocarcinoma (READ) patients in the Cancer Genome Atlas (TCGA) cohort. The patients were dichotomously categorized on the basis of median PIPKIγ value into 2 groups. Subgroups were compared with the use of the log-rank test. * P < .05; ** P < .01; *** P < .001.

Article Snippet: The colorectal cancer tissue microarray used in this study was purchased from Zhuoli Biotech (#COC1504, http://www.zhuolibiotech.com/ , Shanghai, China).

Techniques: Expressing, Western Blot, Control, Microarray

Journal: EBioMedicine

Article Title: Type Iγ phosphatidylinositol phosphate kinase promotes tumor growth by facilitating Warburg effect in colorectal cancer

doi: 10.1016/j.ebiom.2019.05.015

Figure Lengend Snippet: PIPKIγ promotes colorectal cancer cell proliferation in vitro and tumor growth in vivo. (A) COAD samples derived from TCGA cohort was categorized into 2 groups (high versus low) based on median PIPKIγ value. Gene set enrichment analysis (GSEA) was performed to discover the difference between 2 groups. False discovery rate (FDR) was set at 0.25. NES represents normalized enrichment score. (B) Validation of pan-PIPKIγ knockdown and ectopic expression of mutant-PIPKIγ_i2 (resistant to PIPKIγ shRNA) in SW480 and LOVO cells using Western blotting. (C, D) The influence of PIPKIγ on colorectal cancer in vitro cell proliferation was determined by CCK-8 (C) and colony formation (D) assays, respectively. (E) sh-Ctrl, sh-PIPKIγ-1, and sh-PIPKIγ-1 + mPIPKIγ1_i2 SW480 cells were injected subcutaneously into the left forelimb of nude mice ( n = 6 per group). Four weeks later, mice were sacrificed and tumor weights in each group were shown. (F) IHC analysis of PIPKIγ and PCNA expression from indicated subcutaneous xenograft. * P < .05 and ** P < .01.

Article Snippet: The colorectal cancer tissue microarray used in this study was purchased from Zhuoli Biotech (#COC1504, http://www.zhuolibiotech.com/ , Shanghai, China).

Techniques: In Vitro, In Vivo, Derivative Assay, Biomarker Discovery, Knockdown, Expressing, Mutagenesis, shRNA, Western Blot, CCK-8 Assay, Injection